PDGF Receptor alpha + beta (phospho Tyr572/Tyr574) antibody

Cat. No. GTX25443

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse
Package	50 μl ($399)

See all PDGF Receptor alpha products

Datasheet
Document

View product citations for antibody GTX25443 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:100-1:500

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PDGFRá that contains tyrosines 572 and 574 and PDGFRâ tyrosines 579 and 581. The sequence is conserved in PDGFRá mouse, rat and frog.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Platelet-derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo- or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF-AA, -BB or -AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation. PDGFR alpha tyrosines 572 and 574 (579 and 581 in PDGFR beta) are autophosphorylated in the activated receptor, and bind and activate Src family kinases.

Research Area

DATA IMAGES

GTX25443 ICC/IF Image

ICC/IF analysis of MCF7 cells treated with PDGF (50 ng/mL for 10 min) using GTX25443 PDGF Receptor alpha + beta (phospho Tyr572/Tyr574) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes

GTX25443 WB Image

WB analysis of NIH3T3 stimulated with 50ng/mL PDGF-BB for 10 mins (Lane 2-5) using GTX25443 PDGF Receptor alpha + beta (phospho Tyr572/Tyr574) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine containing peptide(Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

50 μl

$ 399

Qty

PDGF Receptor alpha + beta (phospho Tyr572/Tyr574) antibody

Cat. No. GTX25443

Host

Clonality

Isotype

Application

Reactivity