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PDGF Receptor alpha (phospho Tyr742) antibody

Anti-PDGF Receptor alpha (phospho Tyr742) antibody used in Western Blot (WB). GTX25452

Cat No. GTX25452

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Mouse
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.35-1.0 μg/ml
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PDGFR-alpha that contains tyrosine 742.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

platelet derived growth factor receptor alpha , CD140A , PDGFR-2 , PDGFR2

Cellular Localization

Cell membrane,Golgi apparatus

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-PDGF Receptor alpha (phospho Tyr742) antibody used in Western Blot (WB). GTX25452

GTX25452 WB Image

WB (peptide competition) analysis of NIH3T3 cells stimulated with PDGF (Lane 2-5) using GTX25452 PDGF Receptor alpha (phospho Tyr742) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphotyrosine containing peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf