PDGF Receptor alpha (phospho Tyr849) + PDGF Receptor beta (phospho Tyr857) antibody [HL3345]

Specificity test was performed by coating the plate with recombinant PDGFRA non-phospho peptide, PDGFRA (phospho Tyr849) peptide, PDGFRB non-phospho peptide, and PDGFRB (phospho Tyr857) peptide (250 ng/mL). Coated protein was probed with PDGF Receptor alpha (phospho Tyr849) + PDGF Receptor beta (phospho Tyr857) antibody [HL3345] (GTX641117) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with PDGF Receptor alpha (phospho Tyr849) + PDGF Receptor beta (phospho Tyr857) antibody [HL3345] (GTX641117) diluted at 1:1000 and PDGF Receptor beta antibody (GTX133587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.