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PDGF Receptor beta antibody [PR7212]

Cat. No. GTX42416

Reference 9

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PR7212

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Depletion

Reactivity

 Human, Monkey, Baboon, Golden Syrian Hamster
Package
50 μg ($459)
See all PDGF Receptor beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 25μg/ml
IP 25μg/ml
Depletion Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Product Note

This antibody is specific for human PDGF receptor beta protein, and it does not cross react with human PDGF receptor alpha protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human skin fibroblast cell membrane extracts.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet derived growth factor receptor beta , CD140B , IBGC4 , IMF1 , JTK12 , KOGS , PDGFR , PDGFR-1 , PDGFR1 , PENTT

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Cytoplasmic vesicle

Background

The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Aug 2017]

Database

Research Area

REFERENCE

Clone Reference

Gilbertson DG et al. J Biol Chem. 2001; 276 (29):27406-14 Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds to PDGF alpha and beta receptor.
Application : IP
Reactivity : Golden Syrian Hamster
Ascoli V et al. Acta Cytol. 1995; 39 (4):613-22 Platelet-derived growth factor receptor immunoreactivity in mesothelioma and nonneoplastic mesothelial cells in serous effusions.
Wang J et al. Cancer Res. 1994; 54 (2):560-4 Cell proliferation in human soft tissue tumors correlates with platelet-derived growth factor B chain expression: an immunohistochemical and in situ hybridization study.
Application : IHC-P
Reactivity : Human
Palman C et al. Lab Invest. 1992; 66 (1):108-15 Platelet-derived growth factor receptor (beta-subunit) immunoreactivity in soft tissue tumors.
Krane JF et al. J Invest Dermatol. 1991; 96 (6):983-6 Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis.
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Clone Reference
Gilbertson DG et al. J Biol Chem. 2001; 276 (29):27406-14 Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds to PDGF alpha and beta receptor.
Application : IP
Reactivity : Golden Syrian Hamster
Ascoli V et al. Acta Cytol. 1995; 39 (4):613-22 Platelet-derived growth factor receptor immunoreactivity in mesothelioma and nonneoplastic mesothelial cells in serous effusions.
Wang J et al. Cancer Res. 1994; 54 (2):560-4 Cell proliferation in human soft tissue tumors correlates with platelet-derived growth factor B chain expression: an immunohistochemical and in situ hybridization study.
Application : IHC-P
Reactivity : Human
Palman C et al. Lab Invest. 1992; 66 (1):108-15 Platelet-derived growth factor receptor (beta-subunit) immunoreactivity in soft tissue tumors.
Krane JF et al. J Invest Dermatol. 1991; 96 (6):983-6 Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis.
Franklin WA et al. Cancer Res. 1990; 50 (19):6344-8 In situ distribution of the beta-subunit of platelet-derived growth factor receptor in nonneoplastic tissue and in soft tissue tumors.
Application : IHC-Fr
Reactivity : Human
Seifert RA et al. J Biol Chem. 1989; 264 (15):8771-8 Two different subunits associate to create isoform-specific platelet-derived growth factor receptors.
Application : IP
Reactivity : Human
Hart CE et al. Science. 1988; 240 (4858):1529-31 Two classes of PDGF receptor recognize different isoforms of PDGF.
Hart CE et al. J Biol Chem. 1987; 262 (22):10780-5 Synthesis, phosphorylation, and degradation of multiple forms of the platelet-derived growth factor receptor studied using a monoclonal antibody.
Application : WB, IP, Depletion
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459