*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
PDGF treated NIH-3T3
Human, Bovine(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.12 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide corresponding to residues around human PDGF Receptor beta (phospho Tyr751). The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
platelet derived growth factor receptor beta , CD140B , IBGC4 , IMF1 , JTK12 , KOGS , PDGFR , PDGFR-1 , PDGFR1 , PENTT
Membrane; Single-pass type I membrane protein
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq]
GTX133525 WB Image
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor beta (phospho Tyr751) antibody (GTX133525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX133525 ICC/IF Image
PDGF Receptor beta (phospho Tyr751) antibody detects PDGF Receptor beta (phospho Tyr751) protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Mock and treated NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDGF Receptor beta (phospho Tyr751) protein stained by PDGF Receptor beta (phospho Tyr751) antibody (GTX133525) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.