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PDGF Receptor beta (phospho Tyr751) antibody

Anti-PDGF Receptor beta (phospho Tyr751) antibody used in Western Blot (WB). GTX133525
Anti-PDGF Receptor beta (phospho Tyr751) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133525

Cat No. GTX133525

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

124 kDa. ( Note )

Positive Control

PDGF treated NIH-3T3

Predict Reactivity

Human, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human PDGF Receptor beta (phospho Tyr751). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

platelet derived growth factor receptor beta,CD140B,IBGC4,IMF1,JTK12,KOGS,PDGFR,PDGFR-1,PDGFR1,PENTT

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PDGF Receptor beta (phospho Tyr751) antibody used in Western Blot (WB). GTX133525

GTX133525 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor beta (phospho Tyr751) antibody (GTX133525) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDGF Receptor beta (phospho Tyr751) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133525

GTX133525 ICC/IF Image

PDGF Receptor beta (phospho Tyr751) antibody detects PDGF Receptor beta (phospho Tyr751) protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Mock and treated NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDGF Receptor beta (phospho Tyr751) protein stained by PDGF Receptor beta (phospho Tyr751) antibody (GTX133525) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169