GeneTex

  • Country / Location Selection

United States (US)

PDGF beta antibody

Anti-PDGF beta antibody used in Western Blot (WB). GTX54575
Anti-PDGF beta antibody used in IHC (Paraffin sections) (IHC-P). GTX54575
Anti-PDGF beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54575
Anti-PDGF beta antibody used in IHC (Paraffin sections) (IHC-P). GTX54575

Cat. No. GTX54575

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PDGF beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-241 of human PDGFB (NP_002599.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887865

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

platelet derived growth factor subunit B , IBGC5 , PDGF-2 , PDGF2 , SIS , SSV , c-sis

Cellular Localization

Secreted

Background

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-PDGF beta antibody used in Western Blot (WB). GTX54575

GTX54575 WB Image

WB analysis of various cell lysates using GTX54575 PDGF beta antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PDGF beta antibody used in IHC (Paraffin sections) (IHC-P). GTX54575

GTX54575 IHC-P Image

IHC-P analysis of human placenta tissue using GTX54575 PDGF beta antibody.
Dilution : 1:150
Antigen retrieval : 10 mM citrate buffer, pH 6.0

Anti-PDGF beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54575

GTX54575 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54575 PDGF beta antibody.
Dilution : 1:150
Blue : DAPI

Anti-PDGF beta antibody used in IHC (Paraffin sections) (IHC-P). GTX54575

GTX54575 IHC-P Image

IHC-P analysis of rat heart tissue using GTX54575 PDGF beta antibody.
Dilution : 1:150
Antigen retrieval : 10 mM citrate buffer, pH 6.0

REFERENCE

Application Reference

Yang R et al. Cell Commun Signal 2024; 22 (1) : 44 Egr-1 is a key regulator of the blood-brain barrier damage induced by meningitic Escherichia coli.
Wang S et al. Cell Mol Life Sci 2017; (Epub) Platelet-derived growth factor receptor beta identifies mesenchymal stem cells with enhanced engraftment to tissue injury and pro-angiogenic property.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for PDGF beta antibody (GTX54575). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399