APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver
Predict Reactivity
Rat, Rabbit, Bovine, Chicken, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PDI. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Prolyl 4-Hydroxylase Subunit Beta,Clcrp1,Dsi,Erba2L,Git,P4Hbeta,Pdi,Pdia1,Phdb,Po4Db,Po4Hb,Prohb,P4Hb
Cellular Localization
Endoplasmic reticulum lumen , Melanosome , Cell membrane; Peripheral membrane protein (Potential)
Background
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101468 IHC-P Image
PDI antibody [N1N3] detects PDI protein at cytosol and membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PDI antibody [N1N3] (GTX101468) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101468 ICC/IF Image
PDI antibody [N1N3] detects PDI protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDI protein stained by PDI antibody [N1N3] (GTX101468) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
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GTX101468 WB Image
Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
7.5% SDS PAGE
GTX101468 diluted at 1:500
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GTX101468 WB Image
Sample (20 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX101468 diluted at 1:10000
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GTX101468 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using PDI(GTX101468) antibody at 1:200 dilution.
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GTX101468 IHC-P Image
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using PDI(GTX101468) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
