PDI antibody [N1N3]

Cat No. GTX101468

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, EM
Reactivity	Human, Mouse
Package	100 μl ($329), 25 μl ($169)

See all PDI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
EM	Assay dependent

Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241147

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prolyl 4-hydroxylase subunit beta , CLCRP1 , DSI , ERBA2L , GIT , P4Hbeta , PDI , PDIA1 , PHDB , PO4DB , PO4HB , PROHB

Cellular Localization

Endoplasmic reticulum lumen , Melanosome , Cell membrane; Peripheral membrane protein (Potential)

Background

This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq]

Database

Gene ID: 5034 P4HB
UniProt: P07237 P4HB

Research Area

DATA IMAGES

GTX101468 IHC-P Image

PDI antibody [N1N3] detects PDI protein at cytosol and membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PDI antibody [N1N3] (GTX101468) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101468 ICC/IF Image

PDI antibody [N1N3] detects PDI protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDI protein stained by PDI antibody [N1N3] (GTX101468) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101468 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:H1299
7.5% SDS PAGE
GTX101468 diluted at 1:500

GTX101468 WB Image

Sample (20 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX101468 diluted at 1:10000

GTX101468 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using PDI(GTX101468) antibody at 1:200 dilution.

GTX101468 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H520 xenograft, using PDI(GTX101468) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min