FACS analysis of K562 cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of rat epithelial cells using GTX25484 PDI antibody [RL77].

FACS analysis of 3T3 cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of MDCK cells using GTX25484 PDI antibody [RL77].
Green : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature

WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX25484 PDI antibody [RL77].
Dilution : 1:1000

FACS analysis of Hela cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of HMVEC Cells using GTX25484 PDI antibody [RL77].
Green : Primary antibody
Blue : cell nucleus

ICC/IF analysis of NS-1 Cells using GTX25484 PDI antibody [RL77].
Green : Primary antibody
Blue : cell nucleus

IHC-P analysis of human tonsil tissue using GTX25484 PDI antibody [RL77].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

IHC-P analysis of human colon carcinoma tissue using GTX25484 PDI antibody [RL77].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

ICC/IF analysis of A549 cells using GTX25484 PDI antibody [RL77].
Green : Primary antibody
Blue : cell nucleus

IHC-P analysis of human liver tissue using GTX25484 PDI antibody [RL77].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200