PDI antibody [RL90]

FACS analysis of NIH/3T3 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of A549 cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei

FACS analysis of K562 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

IHC-P analysis of human tonsil tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

FACS analysis of Hela cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

IHC-P analysis of human colon tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

IHC-P analysis of human lung adenocarcinoma tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

ICC/IF analysis of p19 Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei

ICC/IF analysis of NS-1 Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei

ICC/IF analysis of U2OS cells using GTX22792 PDI antibody [RL90].
Red : Primary antibody
Green : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature
Dilution : 1:75 for at least 1 hour at room temperature

ICC/IF analysis of HMVEC Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei

ICC/IF analysis of MDCK cells using GTX22792 PDI antibody [RL90].
Permeabilization : 0.1% Triton X-100
Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature

WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX22792 PDI antibody [RL90].
Dilution : 1:1000

ICC/IF analysis of NIH-3T3 cells using GTX22792 PDI antibody [RL90].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature
Dilution : 1:75 for at least 1 hour at room temperature