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PDI antibody [RL90]

Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792
Anti-PDI antibody [RL90] used in Western Blot (WB). GTX22792
Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792
Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792
Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792
Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792
Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792
Anti-PDI antibody [RL90] used in IHC (Paraffin sections) (IHC-P). GTX22792
Anti-PDI antibody [RL90] used in IHC (Paraffin sections) (IHC-P). GTX22792
Anti-PDI antibody [RL90] used in Flow cytometry (FACS). GTX22792
Anti-PDI antibody [RL90] used in IHC (Paraffin sections) (IHC-P). GTX22792
Anti-PDI antibody [RL90] used in Flow cytometry (FACS). GTX22792
Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792
Anti-PDI antibody [RL90] used in Flow cytometry (FACS). GTX22792

Cat. No. GTX22792

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RL90

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IM, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Dog, Hamster, Pig, Rhesus Monkey, Primate
Package
100 μl ($399)
See all PDI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:2000
ICC/IF 1:100
IHC-P 1:100
IHC-Fr 1:100
FACS 0.5-1 μg/test
IP Assay dependent
IM Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

buffer, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Purified rat PDI protein.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384853

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prolyl 4-hydroxylase subunit beta , PDI , PDIR

Cellular Localization

Endoplasmic reticulum,Cell membrane

Background

may be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792

GTX22792 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX22792 PDI antibody [RL90].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature
Dilution : 1:75 for at least 1 hour at room temperature

Anti-PDI antibody [RL90] used in Western Blot (WB). GTX22792

GTX22792 WB Image

WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX22792 PDI antibody [RL90].
Dilution : 1:1000

Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792

GTX22792 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX22792 PDI antibody [RL90].
Permeabilization : 0.1% Triton X-100
Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature

Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792

GTX22792 ICC/IF Image

ICC/IF analysis of HMVEC Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei

Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792

GTX22792 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX22792 PDI antibody [RL90].
Red : Primary antibody
Green : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature
Dilution : 1:75 for at least 1 hour at room temperature

Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792

GTX22792 ICC/IF Image

ICC/IF analysis of NS-1 Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei

Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792

GTX22792 ICC/IF Image

ICC/IF analysis of p19 Cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei

Anti-PDI antibody [RL90] used in IHC (Paraffin sections) (IHC-P). GTX22792

GTX22792 IHC-P Image

IHC-P analysis of human lung adenocarcinoma tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-PDI antibody [RL90] used in IHC (Paraffin sections) (IHC-P). GTX22792

GTX22792 IHC-P Image

IHC-P analysis of human colon tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-PDI antibody [RL90] used in Flow cytometry (FACS). GTX22792

GTX22792 FACS Image

FACS analysis of Hela cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PDI antibody [RL90] used in IHC (Paraffin sections) (IHC-P). GTX22792

GTX22792 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22792 PDI antibody [RL90].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-PDI antibody [RL90] used in Flow cytometry (FACS). GTX22792

GTX22792 FACS Image

FACS analysis of K562 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PDI antibody [RL90] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22792

GTX22792 ICC/IF Image

ICC/IF analysis of A549 cells using GTX22792 PDI antibody [RL90].
Green : Primary antibody
Blue : Nuclei

Anti-PDI antibody [RL90] used in Flow cytometry (FACS). GTX22792

GTX22792 FACS Image

FACS analysis of NIH/3T3 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

Application Reference

Yating Cheng et al. Pakistan Journal of Zoology 2019; (Epub) http://researcherslinks.com/current-issues/New-Strategy-for-Expression-of-Recombinant/20/1/2539/html.
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399