APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:20 - 1:100 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 329-508 of human P4HB (NP_000909.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
prolyl 4-hydroxylase subunit beta , CLCRP1 , DSI , ERBA2L , GIT , P4Hbeta , PDI , PDIA1 , PHDB , PO4DB , PO4HB , PROHB
Cellular Localization
Endoplasmic reticulum,Cell membrane
Background
This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX55742 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55742 PDI antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX55742 WB Image
WB analysis of various sample lysates using GTX55742 PDI antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX55742 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX55742 PDI antibody.
Blue : DAPI
Dilution : 1:400
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GTX55742 IP Image
IP analysis of SW620 cell lysate using GTX55742 PDI antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:500
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GTX55742 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX55742 PDI antibody.
Blue : DAPI
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REFERENCE
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REVIEW
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