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PDIA6 antibody [N1N3]

Anti-PDIA6 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX121275
Anti-PDIA6 antibody [N1N3] used in Immunoprecipitation (IP). GTX121275
Anti-PDIA6 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX121275
Anti-PDIA6 antibody [N1N3] used in Western Blot (WB). GTX121275

Cat. No. GTX121275

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PDIA6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDIA6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10726090

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein disulfide isomerase family A member 6 , ERP5 , P5 , TXNDC7

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (Hayano and Kikuchi, 1995 [PubMed 7590364]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PDIA6 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX121275

GTX121275 IHC-P Image

PDIA6 antibody [N1N3] detects PDIA6 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PDIA6 antibody [N1N3] (GTX121275) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDIA6 antibody [N1N3] used in Immunoprecipitation (IP). GTX121275

GTX121275 IP Image

Immunoprecipitation of PDIA6 protein from 293T whole cell extracts using 5 μg of PDIA6 antibody [N1N3] (GTX121275).
Western blot analysis was performed using PDIA6 antibody [N1N3] (GTX121275).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PDIA6 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX121275

GTX121275 IHC-P Image

PDIA6 antibody [N1N3] detects PDIA6 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
PDIA6 antibody [N1N3] (GTX121275) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDIA6 antibody [N1N3] used in Western Blot (WB). GTX121275

GTX121275 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX121275 diluted at 1:1000

REFERENCE

Application Reference

Tsuchiya Y et al. J Cell Biol 2018; (Epub) ire1-xbp1 pathway regulates oxidative pro* folding in pancreatic β cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169