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PDIA6 antibody

Anti-PDIA6 antibody used in Western Blot (WB). GTX11432
Anti-PDIA6 antibody used in Western Blot (WB). GTX11432
Anti-PDIA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11432

Cat. No. GTX11432

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Pig
Package
50 μl ($399)
See all PDIA6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250
ICC/IF 1:250
FACS 1:20
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues D(476) I D L S D V E L D D L G K D E L(492) of human P5.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein disulfide isomerase family A member 6 , ERP5 , P5 , TXNDC7

Cellular Localization

Cell membrane

Background

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-PDIA6 antibody used in Western Blot (WB). GTX11432

GTX11432 WB Image

WB analysis of membrane enriched extracts (30 μg lysate) of A549 (Lane 1), A-431 (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), K-562 (Lane 5), Caco-2 (Lane 6), Hep G2 (Lane 7) and COLO 205 (Lane 8) using GTX11432 PDIA6 antibody.
Dilution : 1:250

Anti-PDIA6 antibody used in Western Blot (WB). GTX11432

GTX11432 WB Image

WB analysis of HT-1080 cell lysate using GTX11432 PDIA6 antibody.

Anti-PDIA6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11432

GTX11432 ICC/IF Image

ICC/IF analysis of A549 cells using GTX11432 PDIA6 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399