GeneTex
United States (US)

PDIR antibody [N1N3]

GTX115511_40212_20150612_IHC_M_18121410_787.jpg
GTX115511_40212_IFA_18121410_839.jpg
GTX115511_WB_18121410_861.jpg
GTX115511_40212_IHC_18121410_968.jpg

Cat No. GTX115511

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

A549 , HepG2 , HCT116

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDIR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Disulfide Isomerase Family A Member 5,Pdir,Pdia5

Cellular Localization

Endoplasmic reticulum lumen

Database

DATA IMAGES
GTX115511_40212_20150612_IHC_M_18121410_787.jpg

GTX115511 IHC-P Image

PDIR antibody [N1N3] detects PDIR protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
PDIR antibody [N1N3] (GTX115511) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115511_40212_IFA_18121410_839.jpg

GTX115511 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using PDIR(GTX115511) antibody at 1:200 dilution.

GTX115511_WB_18121410_861.jpg

GTX115511 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX115511 diluted at 1:1000

GTX115511_40212_IHC_18121410_968.jpg

GTX115511 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PDIR(GTX115511) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169