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PDK3 antibody [N1C2]

Overexpression
Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286
Anti-PDK3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104286
Anti-PDK3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104286
Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286
Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286

Cat. No. GTX104286

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Frog, Squirrel
Package
100 μl ($399),
25 μl ($169)
See all PDK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

mouse liver , HepG2 , PDK3-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951161

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate dehydrogenase kinase 3 , CMTX6 , GS1-358P8.4

Cellular Localization

Mitochondrion matrix

Background

Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Database

Research Area

DATA IMAGES

Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286

GTX104286 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDK3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104286

GTX104286 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using PDK3(GTX104286) antibody at 1:200 dilution.

Anti-PDK3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104286

GTX104286 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using PDK3(GTX104286) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286

GTX104286 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000.

Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286

GTX104286 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jonus HC et al. Biomed Pharmacother 2020; 121 : 109648 Thiamine mimetics sulbutiamine and benfotiamine as a nutraceutical approach to anticancer therapy.
Application : WB
Reactivity : Human
Al-Attar R et al. Cryobiology 2019; 86 : 10-18 Metabolic reorganization in winter: Regulation of pyruvate dehydrogenase (PDH) during long-term freezing and anoxia.
Application : WB
Reactivity : Frog
Wijenayake S et al. Journal of Thermal Biology 2017; 69 : 199-205 Regulation of pyruvate dehydrogenase (PDH) in the hibernating ground squirrel, (Ictidomys tridecemlineatus).
Application : WB
Reactivity : Squirrel
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169