GeneTex
  • Country / Location Selection

United States (US)

PDK3 antibody [N1C2]

Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286
Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286
Anti-PDK3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104286
Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286
Anti-PDK3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104286

Cat No. GTX104286

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Frog, Squirrel
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HepG2 , mouse liver , PDK3-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

pyruvate dehydrogenase kinase 3 , CMTX6 , GS1-358P8.4

Cellular Localization

Mitochondrion matrix

Background

Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Database

Research Area

DATA IMAGES
Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286

GTX104286 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000.

Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286

GTX104286 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDK3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104286

GTX104286 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using PDK3(GTX104286) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDK3 antibody [N1C2] used in Western Blot (WB). GTX104286

GTX104286 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] (GTX104286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PDK3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104286

GTX104286 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using PDK3(GTX104286) antibody at 1:200 dilution.

REFERENCE
REVIEW
There are currently no reviews for PDK3 antibody [N1C2] (GTX104286). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169