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PDK3 antibody

Anti-PDK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65965
Anti-PDK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65965
Anti-PDK3 antibody used in Western Blot (WB). GTX65965

Cat No. GTX65965

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pyruvate dehydrogenase kinase 3 , CMTX6 , GS1-358P8.4

Cellular Localization

Mitochondrion matrix

Background

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-PDK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65965

GTX65965 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX65965 PDK3 antibody.
Dilution : 1:100

Anti-PDK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX65965

GTX65965 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX65965 PDK3 antibody.
Dilution : 1:100

Anti-PDK3 antibody used in Western Blot (WB). GTX65965

GTX65965 WB Image

WB analysis of various sample lysates using GTX65965 PDK3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379