GeneTex
United States (US)

PDLIM1 antibody, C-term

GTX17022_419_IHC-P_18121410_492.jpg
GTX17022_1449_WB_18121410_293.jpg

Cat No. GTX17022

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX17022-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TPPEGYEVVTVFPK, from the C Terminus of the protein sequence according to NP_066272.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pdz And Lim Domain 1,Clim1,Clp-36,Clp36,Hel-S-112,Hclim1,Pdlim1

Cellular Localization

Associates with the actin stress fibers.

Background

This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012]

Database

Research Area

DATA IMAGES
GTX17022_419_IHC-P_18121410_492.jpg

GTX17022 IHC-P Image

IHC-P analysis of human placenta using GTX17022 PDLIM1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml

GTX17022_1449_WB_18121410_293.jpg

GTX17022 WB Image

WB analysis of human lung lysate using GTX17022 PDLIM1 antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer