GeneTex
United States (US)

PDLIM4 antibody [C2C3], C-term

GTX106353_39701_IFA_19011506_766.jpg
GTX106353_WB_19011506_851.jpg

Cat No. GTX106353

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PDLIM4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RIL antibody, PDLIM4 antibody, LIM protein RIL antibody, LIM domain protein antibody, PDZ and LIM domain protein 4 antibody, reversion-induced LIM protein antibody, enigma homolog antibody, PDZ and LIM domain 4 antibody

Background

This gene encodes a protein which may be involved in bone development. Mutations in this gene are associated with susceptibility to osteoporosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106353_39701_IFA_19011506_766.jpg

GTX106353 ICC/IF Image

PDLIM4 antibody [C2C3], C-term detects PDLIM4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PDLIM4 protein stained by PDLIM4 antibody [C2C3], C-term (GTX106353) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX106353_WB_19011506_851.jpg

GTX106353 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX106353 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf