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PDPK1 / PDK1 antibody

Orthogonal
Anti-PDPK1 / PDK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50366
Anti-PDPK1 / PDK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50366
Anti-PDPK1 / PDK1 antibody used in Western Blot (WB). GTX50366

Cat. No. GTX50366

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all PDPK1 / PDK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.239~243 (A-N-S-F-V) derived from human PDPK1 / PDK1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-phosphoinositide dependent protein kinase 1 , PDK1 , PDPK2 , PDPK2P , PRO0461

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-PDPK1 / PDK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50366

GTX50366 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50366 PDPK1 / PDK1 antibody.

Anti-PDPK1 / PDK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50366

GTX50366 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50366 PDPK1 / PDK1 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-PDPK1 / PDK1 antibody used in Western Blot (WB). GTX50366

GTX50366 WB Image

Orthogonal

WB analysis of extracts from MCF, 293, and PC12 cells using GTX50366 PDPK1 / PDK1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399