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PDPK1 / PDK1 (phospho Ser241) antibody

Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in Western Blot (WB). GTX50093
Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in IHC (Paraffin sections) (IHC-P). GTX50093
Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50093

Cat No. GTX50093

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from human PDPK1 / PDK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

3-phosphoinositide dependent protein kinase 1 , PDK1 , PDPK2 , PDPK2P , PRO0461

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in Western Blot (WB). GTX50093

GTX50093 WB Image

WB analysis of extracts from 293 and PC12 cells untreated or treated with EGF using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.

Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in IHC (Paraffin sections) (IHC-P). GTX50093

GTX50093 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50093

GTX50093 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf