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PDPK1 / PDK1 (phospho Ser241) antibody

Orthogonal
Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in Western Blot (WB). GTX50093
Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in IHC (Paraffin sections) (IHC-P). GTX50093
Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50093

Cat. No. GTX50093

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all PDPK1 / PDK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from human PDPK1 / PDK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11174223

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-phosphoinositide dependent protein kinase 1 , PDK1 , PDPK2 , PDPK2P , PRO0461

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in Western Blot (WB). GTX50093

GTX50093 WB Image

Orthogonal

WB analysis of extracts from 293 and PC12 cells untreated or treated with EGF using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.

Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in IHC (Paraffin sections) (IHC-P). GTX50093

GTX50093 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-PDPK1 / PDK1 (phospho Ser241) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50093

GTX50093 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50093 PDPK1 / PDK1 (phospho Ser241) antibody.

REFERENCE

Application Reference

Cao W et al. Sci Rep 2015; 5 (Epub) : 8477 homoharringtonine induces apoptosis and inhibits stat3 via il-6/jak1/stat3 signal pathway in *-resistant lung cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399