APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:20000 |
| 1:50-1:100 |
| 1:50 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human recombinant protein of human PDX1 (NP_000200) produced in E.coli.
Purification
Purified by affinity chromatography
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
pancreatic and duodenal homeobox 1 , GSF , IDX-1 , IPF1 , IUF1 , MODY4 , PAGEN1 , PDX-1 , STF-1
Cellular Localization
Nucleus,Cytoplasm, cytosol
Background
Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
Database
Research Area
DATA IMAGES
![Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919](/templates/default/js/lightbox/images/loading.gif "Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919")
|
GTX83919 IHC-P Image
IHC-P analysis of human pancreas carcinoma tissue using GTX83919 PDX1 antibody [2A12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
|
|
|
GTX83919 ICC/IF Image
ICC/IF analysis of HeLa cells transiently transfected with PDX1 plasmid using GTX83919 PDX1 antibody [2A12].
|
|
|
GTX83919 ICC/IF Image
ICC/IF analysis of HT29 cells using GTX83919 PDX1 antibody [2A12].
|
|
|
GTX83919 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX83919 PDX1 antibody [2A12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
|
|
|
GTX83919 WB Image
WB analysis of various samples using GTX83919 PDX1 antibody [2A12].
Loading : 10 ug per lane
Dilution : 1:200
|
|
|
GTX83919 WB Image
WB analysis of HEK293T cells transfected with PDX1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83919 PDX1 antibody [2A12].
Loading : 5 ug per lane
|
REFERENCE
There are currently no references for PDX1 antibody [2A12] (GTX83919). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PDX1 antibody [2A12] (GTX83919). Be the first to share your experience with this product.
![Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919](/upload/website/prouct_img/small/GTX83919/GTX83919_2019_IHC-P_w_23061420_853.webp "Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919")
![Anti-PDX1 antibody [2A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83919](/upload/website/prouct_img/small/GTX83919/GTX83919_878_ICCIF_w_23061420_725.webp "Anti-PDX1 antibody [2A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83919")
![Anti-PDX1 antibody [2A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83919](/upload/website/prouct_img/small/GTX83919/GTX83919_879_ICCIF_w_23061420_902.webp "Anti-PDX1 antibody [2A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83919")
![Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919](/upload/website/prouct_img/small/GTX83919/GTX83919_2020_IHC-P_w_23061420_793.webp "Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919")
![Anti-PDX1 antibody [2A12] used in Western Blot (WB). GTX83919](/upload/website/prouct_img/small/GTX83919/GTX83919_3474_WB_w_23061420_736.webp "Anti-PDX1 antibody [2A12] used in Western Blot (WB). GTX83919")
![Anti-PDX1 antibody [2A12] used in Western Blot (WB). GTX83919](/upload/website/prouct_img/small/GTX83919/GTX83919_4003_WB_w_23061420_906.webp "Anti-PDX1 antibody [2A12] used in Western Blot (WB). GTX83919")
![Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919](/upload/website/prouct_img/normal/GTX83919/GTX83919_2019_IHC-P_w_23061420_853.webp "Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919")
![Anti-PDX1 antibody [2A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83919](/upload/website/prouct_img/normal/GTX83919/GTX83919_878_ICCIF_w_23061420_725.webp "Anti-PDX1 antibody [2A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83919")
![Anti-PDX1 antibody [2A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83919](/upload/website/prouct_img/normal/GTX83919/GTX83919_879_ICCIF_w_23061420_902.webp "Anti-PDX1 antibody [2A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83919")
![Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919](/upload/website/prouct_img/normal/GTX83919/GTX83919_2020_IHC-P_w_23061420_793.webp "Anti-PDX1 antibody [2A12] used in IHC (Paraffin sections) (IHC-P). GTX83919")
![Anti-PDX1 antibody [2A12] used in Western Blot (WB). GTX83919](/upload/website/prouct_img/normal/GTX83919/GTX83919_3474_WB_w_23061420_736.webp "Anti-PDX1 antibody [2A12] used in Western Blot (WB). GTX83919")
![Anti-PDX1 antibody [2A12] used in Western Blot (WB). GTX83919](/upload/website/prouct_img/normal/GTX83919/GTX83919_4003_WB_w_23061420_906.webp "Anti-PDX1 antibody [2A12] used in Western Blot (WB). GTX83919")