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PDX1 antibody [5A5]

Anti-PDX1 antibody [5A5] used in Western Blot (WB). GTX60573
Anti-PDX1 antibody [5A5] used in Flow cytometry (FACS). GTX60573
Anti-PDX1 antibody [5A5] used in Western Blot (WB). GTX60573
Anti-PDX1 antibody [5A5] used in ELISA (ELISA). GTX60573

Cat. No. GTX60573

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5A5

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all PDX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PDX1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pancreatic and duodenal homeobox 1 , GSF , IDX-1 , IPF1 , IUF1 , MODY4 , PAGEN1 , PDX-1 , STF-1

Cellular Localization

Nucleus,Cytoplasm, cytosol

Background

The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PDX1 antibody [5A5] used in Western Blot (WB). GTX60573

GTX60573 WB Image

WB analysis of human PDX1 (AA: 39-283) recombinant protein using GTX60573 PDX1 antibody [5A5].

Anti-PDX1 antibody [5A5] used in Flow cytometry (FACS). GTX60573

GTX60573 FACS Image

FACS analysis of Jurkat cells using GTX60573 PDX1 antibody [5A5].
Green : PDX1
Red : negative control

Anti-PDX1 antibody [5A5] used in Western Blot (WB). GTX60573

GTX60573 WB Image

WB analysis of HEK293 (1) and PDX1 (AA: 39-283)-hIgGFc transfected HEK293 (2) cell lysate using GTX60573 PDX1 antibody [5A5].

Anti-PDX1 antibody [5A5] used in ELISA (ELISA). GTX60573

GTX60573 ELISA Image

ELISA analysis of antigen using GTX60573 PDX1 antibody [5A5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409