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PDX1 antibody [5D5]

Overexpression
Anti-PDX1 antibody [5D5] used in Western Blot (WB). GTX83918
Anti-PDX1 antibody [5D5] used in Western Blot (WB). GTX83918

Cat. No. GTX83918

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D5

Isotype

 IgG1

Application

 WB

Reactivity

 Human
Package
100 μl ($519)
See all PDX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:10000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PDX1 (NP_000200) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pancreatic and duodenal homeobox 1 , GSF , IDX-1 , IPF1 , IUF1 , MODY4 , PAGEN1 , PDX-1 , STF-1

Cellular Localization

Nucleus,Cytoplasm, cytosol

Background

Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

Database

Research Area

DATA IMAGES

Anti-PDX1 antibody [5D5] used in Western Blot (WB). GTX83918

GTX83918 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PDX1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83918 PDX1 antibody [5D5].
Loading : 5 ug per lane

Anti-PDX1 antibody [5D5] used in Western Blot (WB). GTX83918

GTX83918 WB Image

WB analysis of various cell lines using GTX83918 PDX1 antibody [5D5].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519