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PDX1 antibody

Anti-PDX1 antibody used in Western Blot (WB). GTX130034
Anti-PDX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130034
Anti-PDX1 antibody used in Western Blot (WB). GTX130034
Anti-PDX1 antibody used in Western Blot (WB). GTX130034
Anti-PDX1 antibody used in Western Blot (WB). GTX130034
Anti-PDX1 antibody used in Western Blot (WB). GTX130034

Cat No. GTX130034

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , mouse liver , rat liver , AsPC-1 , mouse pancreas

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PDX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pancreatic and duodenal homeobox 1 , GSF , IDX-1 , IPF1 , IUF1 , MODY4 , PAGEN1 , PDX-1 , STF-1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PDX1 antibody used in Western Blot (WB). GTX130034

GTX130034 WB Image

PDX1 antibody detects PDX1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PDX1 antibody (GTX130034) diluted at a dilution of 1:1000.

Anti-PDX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130034

GTX130034 IHC-P Image

PDX1 antibody detects PDX1 protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
PDX1 antibody (GTX130034) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDX1 antibody used in Western Blot (WB). GTX130034

GTX130034 WB Image

PDX1 antibody detects PDX1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with PDX1 antibody (GTX130034) at a dilution of 1:1000.

Anti-PDX1 antibody used in Western Blot (WB). GTX130034

GTX130034 WB Image

PDX1 antibody detects PDX1 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with PDX1 antibody (GTX130034) at a dilution of 1:1000.

Anti-PDX1 antibody used in Western Blot (WB). GTX130034

GTX130034 WB Image

PDX1 antibody detects PDX1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PDX1 antibody (GTX130034) at a dilution of 1:1000.

Anti-PDX1 antibody used in Western Blot (WB). GTX130034

GTX130034 WB Image

PDX1 antibody detects PDX1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with PDX1 antibody (GTX130034) at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169