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ProDynorphin antibody [N1C2]

Anti-ProDynorphin antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113515
Anti-ProDynorphin antibody [N1C2] used in Western Blot (WB). GTX113515
Anti-ProDynorphin antibody [N1C2] used in IHC (Frozen sections) (IHC-Fr). GTX113515
Anti-ProDynorphin antibody [N1C2] used in Western Blot (WB). GTX113515

Cat. No. GTX113515

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ProDynorphin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Molt-4 , Raji , mouse brain , rat brain

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037755

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prodynorphin , ADCA , PENKB , SCA23

Cellular Localization

Secreted

Background

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ProDynorphin antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113515

GTX113515 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PDYN(GTX113515) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ProDynorphin antibody [N1C2] used in Western Blot (WB). GTX113515

GTX113515 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PDYN antibody [N1C2] (GTX113515) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ProDynorphin antibody [N1C2] used in IHC (Frozen sections) (IHC-Fr). GTX113515

GTX113515 IHC-Fr Image

PDYN antibody [N1C2] detects PDYN protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: PDYN stained by PDYN antibody [N1C2] (GTX113515) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ProDynorphin antibody [N1C2] used in Western Blot (WB). GTX113515

GTX113515 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PDYN antibody [N1C2] (GTX113515) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production