GeneTex
United States (US)

PDYN antibody [N1C2]

GTX113515_40128_IHC_18121410_743.jpg
GTX113515_WB_18121410_912.jpg
GTX113515_40128_20171222_WB_M_R_18121410_558.jpg
GTX113515_40128_20180604_IHC-Fr_M_18121410_216.jpg

Cat No. GTX113515

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji , mouse brain , rat brain

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDYN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prodynorphin,Adca,Penkb,Sca23,Pdyn

Cellular Localization

Secreted

Background

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113515_40128_IHC_18121410_743.jpg

GTX113515 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PDYN(GTX113515) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113515_WB_18121410_912.jpg

GTX113515 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
12% SDS PAGE
GTX113515 diluted at 1:1000

GTX113515_40128_20171222_WB_M_R_18121410_558.jpg

GTX113515 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PDYN antibody [N1C2] (GTX113515) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113515_40128_20180604_IHC-Fr_M_18121410_216.jpg

GTX113515 IHC-Fr Image

PDYN antibody [N1C2] detects PDYN protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: PDYN stained by PDYN antibody [N1C2] (GTX113515) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169