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PDZK1 antibody [C1C3]

Anti-PDZK1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX114628
Anti-PDZK1 antibody [C1C3] used in Western Blot (WB). GTX114628

Cat. No. GTX114628

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PDZK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDZK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PDZ domain containing 1 , CAP70 , CLAMP , NHERF-3 , NHERF3 , PDZD1

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.

Database

Research Area

DATA IMAGES

Anti-PDZK1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX114628

GTX114628 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using PDZK1(GTX114628) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PDZK1 antibody [C1C3] used in Western Blot (WB). GTX114628

GTX114628 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX114628 diluted at 1:1000

REFERENCE

Application Reference

Zheng J et al. EBioMedicine 2017; 15 : 62-72 Low level of PDZ domain containing 1 (PDZK1) predicts poor clinical outcome in patients with clear cell renal cell carcinoma.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169