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PECI antibody

Anti-PECI antibody used in Western Blot (WB). GTX122557
Anti-PECI antibody used in IHC (Paraffin sections) (IHC-P). GTX122557
Anti-PECI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122557

Cat. No. GTX122557

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PECI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PECI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171212

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enoyl-CoA delta isomerase 2 , ACBD2 , DRS-1 , DRS1 , HCA88 , PECI , dJ1013A10.3

Background

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-PECI antibody used in Western Blot (WB). GTX122557

GTX122557 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX122557 diluted at 1:5000

Anti-PECI antibody used in IHC (Paraffin sections) (IHC-P). GTX122557

GTX122557 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PECI(GTX122557) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PECI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122557

GTX122557 ICC/IF Image

PECI antibody detects PECI protein at Mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: PECI protein stained by PECI antibody (GTX122557) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169