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PEDF antibody [1C4]

Anti-PEDF antibody [1C4] used in Western Blot (WB). GTX50066

Cat No. GTX50066

Host

Mouse

Clonality

Monoclonal

Clone Name

1C4

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:500 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PEDF (19-418 aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serpin family F member 1 , EPC-1 , OI12 , OI6 , PEDF , PIG35

Cellular Localization

Secreted

Background

This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-PEDF antibody [1C4] used in Western Blot (WB). GTX50066

GTX50066 WB Image

A549 cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

There are currently no references for PEDF antibody [1C4] (GTX50066). Be the first to share your publications with this product.

REVIEW

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SDS
Sodium Azide.pdf