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PEDF antibody [N1C2]

Anti-PEDF antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114097
Anti-PEDF antibody [N1C2] used in Western Blot (WB). GTX114097

Cat. No. GTX114097

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEDF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618544

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family F member 1 , EPC-1 , OI12 , OI6 , PEDF , PIG35

Cellular Localization

Secreted , Melanosome

Background

The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PEDF antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114097

GTX114097 IHC-P Image

PEDF antibody [N1C2] detects PEDF protein at cytoplasm in mouse eye by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
PEDF antibody [N1C2] (GTX114097) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PEDF antibody [N1C2] used in Western Blot (WB). GTX114097

GTX114097 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PEDF antibody [N1C2] (GTX114097) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for PEDF antibody [N1C2] (GTX114097). Be the first to share your publications with this product.

REVIEW

PEDF antibody [N1C2] Cat. No. GTX114097

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 01-Jul-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Conditioned media of human tissue-resident stem cells
Amount used : 5 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1500, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169