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PEDF antibody [N1C2]

Anti-PEDF antibody [N1C2] used in Western Blot (WB). GTX114097
Anti-PEDF antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114097

Cat No. GTX114097

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Rat, Sheep, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEDF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618544

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serpin family F member 1 , EPC-1 , OI12 , OI6 , PEDF , PIG35

Cellular Localization

Secreted , Melanosome

Background

The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PEDF antibody [N1C2] used in Western Blot (WB). GTX114097

GTX114097 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
PEDF antibody
GTX114097 diluted at 1:1000

Anti-PEDF antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX114097

GTX114097 IHC-P Image

PEDF antibody [N1C2] detects PEDF protein at cytoplasm in mouse eye by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
PEDF antibody [N1C2] (GTX114097) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for PEDF antibody [N1C2] (GTX114097). Be the first to share your publications with this product.

REVIEW

PEDF antibody [N1C2] Cat No. GTX114097

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 01-Jul-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Conditioned media of human tissue-resident stem cells
Amount used : 5 μg
Blocking : 3% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1500, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf