WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). 40μg of Y79 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti PEDF (1μg/ml). Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions/concentrations should be determined by the end user.
RIPA lysates from Y79 cells
Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms.
Preservative: 0.05% Sodium Azide; Constituents: Supplied as a liquid in 0.1M Tris glycine, 0.15M sodium chloride, before the addition of glycerol to 30%. pH 7.4
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
His tagged fusion protein corresponding to full length human PEDF (Pigment Epithelial Derived Factor) expressed in baby hamster kidney cells.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Serpin Family F Member 1,Epc-1,Oi12,Oi6,Pedf,Pig35,Serpinf1
Pigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial (RPE) cells, is a neurotrophic protein that induces extensive neuronal differentiation in human Y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts. It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina. PEDF is a potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.