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PEF1 antibody [N2C3]

Anti-PEF1 antibody [N2C3] used in Western Blot (WB). GTX106588
Anti-PEF1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX106588
Anti-PEF1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106588

Cat No. GTX106588

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951170

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

penta-EF-hand domain containing 1 , ABP32 , PEF1A

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1; MIM 114170), sorcin (SRI; MIM 182520), grancalcin (GCA; MIM 607030), and ALG2 (PDCD6; MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PEF1 antibody [N2C3] used in Western Blot (WB). GTX106588

GTX106588 WB Image

Sample(30 ug whole cell lysate)
A:293T
12% SDS PAGE
GTX106588 diluted at 1:1000

Anti-PEF1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX106588

GTX106588 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using PEF1(GTX106588) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PEF1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106588

GTX106588 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Peflin(GTX106588) antibody at 1:200 dilution.

REFERENCE

There are currently no references for PEF1 antibody [N2C3] (GTX106588). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PEF1 antibody [N2C3] (GTX106588). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169