GeneTex
United States (US)

PEF1 antibody [N2C3]

GTX106588_WB_19011506_426.jpg
GTX106588_39959_IHC_19011506_500.jpg
GTX106588_IFA_19011506_445.jpg

Cat No. GTX106588

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ABP32 antibody, PEF1A antibody, PEF1 antibody, peflin antibody, PEF protein with a long N-terminal hydrophobic domain antibody, penta-EF hand domain containing 1 antibody, penta-EF-hand domain containing 1 antibody

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1; MIM 114170), sorcin (SRI; MIM 182520), grancalcin (GCA; MIM 607030), and ALG2 (PDCD6; MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX106588_WB_19011506_426.jpg

GTX106588 WB Image

Sample(30 ug whole cell lysate)
A:293T
12% SDS PAGE
GTX106588 diluted at 1:1000

GTX106588_39959_IHC_19011506_500.jpg

GTX106588 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using PEF1(GTX106588) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106588_IFA_19011506_445.jpg

GTX106588 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Peflin(GTX106588) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169