mPEG3 OVA and Acid PEG3 OVA (1 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PEG (methoxy group) antibody [HL1020] (GTX635825) diluted at 1:128000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant OVA, mPEG24 OVA, mPEG3 OVA, and acid PEG3 OVA (100-1.56 ng/mL). Coated protein was probed with PEG (methoxy group) antibody [HL1020] (GTX635825) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.