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PEG10 antibody [1E2-F12-C12]

Anti-PEG10 antibody [1E2-F12-C12] used in Immunoprecipitation (IP). GTX49210
Anti-PEG10 antibody [1E2-F12-C12] used in Western Blot (WB). GTX49210

Cat No. GTX49210

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1E2-F12-C12

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human
Package
100 μg ($319)
See all PEG10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human PEG10 protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Paternally Expressed 10 , Edr , Hb-1 , Mef3L , Mar2 , Mart2 , Rgag3 , Rtl2 , Sirh1 , Peg10

Cellular Localization

Nucleus. Cytoplasm

Background

This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs), RF1 and RF1/RF2, as well as retroviral-like slippage and pseudoknot elements, which can induce a -1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of -1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Additional isoforms resulting from alternatively spliced transcript variants, as well as from use of upstream non-AUG (CUG) start codon, have been reported for this gene. Increased expression of this gene is associated with hepatocellular carcinomas. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-PEG10 antibody [1E2-F12-C12] used in Immunoprecipitation (IP). GTX49210

GTX49210 IP Image

IP analysis of HeLa lysates using control IgG and PEG10 antibody. The precipitates were detected by the same PEG10 antibody.

Anti-PEG10 antibody [1E2-F12-C12] used in Western Blot (WB). GTX49210

GTX49210 WB Image

WB analysis of HeLa lysate using PEG10 antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319