APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:200 - 1:2000 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human PEG10 (NP_001035242.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
paternally expressed 10 , EDR , HB-1 , MEF3L , Mar2 , Mart2 , RGAG3 , RTL2 , SIRH1
Cellular Localization
Nucleus,Cytoplasm
Background
This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene. [provided by RefSeq, Oct 2014]
Database
Research Area
DATA IMAGES
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GTX64752 WB Image
WB analysis of various sample lysates using GTX64752 PEG10 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX64752 IHC-P Image
IHC-P analysis of rat testis tissue using GTX64752 PEG10 antibody. Dilution : 1:100
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GTX64752 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX64752 PEG10 antibody. Dilution : 1:100
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GTX64752 IHC-P Image
IHC-P analysis of human breast cancer tissue using GTX64752 PEG10 antibody. Dilution : 1:100
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GTX64752 IHC-P Image
IHC-P analysis of human placenta tissue using GTX64752 PEG10 antibody. Dilution : 1:100
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REFERENCE
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REVIEW
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