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PELO antibody [HL2345]

Recombinant antibody
Anti-PELO antibody [HL2345] used in IHC (Whole mount) (IHC-Wm). GTX638548
Anti-PELO antibody [HL2345] used in IHC (Whole mount) (IHC-Wm). GTX638548
Anti-PELO antibody [HL2345] used in Western Blot (WB). GTX638548
Anti-PELO antibody [HL2345] used in Western Blot (WB). GTX638548
Anti-PELO antibody [HL2345] used in Western Blot (WB). GTX638548
Anti-PELO antibody [HL2345] used in Western Blot (WB). GTX638548
Anti-PELO antibody [HL2345] used in IHC (Paraffin sections) (IHC-P). GTX638548
Anti-PELO antibody [HL2345] used in IHC (Paraffin sections) (IHC-P). GTX638548
Anti-PELO antibody [HL2345] used in IHC (Paraffin sections) (IHC-P). GTX638548

Cat. No. GTX638548

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2345

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PELO products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human PELO.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pelota mRNA surveillance and ribosome rescue factor , CGI-17 , PRO1770

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PELO antibody [HL2345] used in IHC (Whole mount) (IHC-Wm). GTX638548

GTX638548 IHC-Wm Image

PELO antibody [HL2345] detects PELO protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: PELO stained by PELO antibody [HL2345] (GTX638548) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-PELO antibody [HL2345] used in IHC (Whole mount) (IHC-Wm). GTX638548

GTX638548 IHC-Wm Image

PELO antibody [HL2345] detects PELO protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: PELO stained by PELO antibody [HL2345] (GTX638548) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-PELO antibody [HL2345] used in Western Blot (WB). GTX638548

GTX638548 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody [HL2345] (GTX638548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PELO antibody [HL2345] used in Western Blot (WB). GTX638548

GTX638548 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody [HL2345] (GTX638548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PELO antibody [HL2345] used in Western Blot (WB). GTX638548

GTX638548 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody [HL2345] (GTX638548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PELO antibody [HL2345] used in Western Blot (WB). GTX638548

GTX638548 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PELO antibody [HL2345] (GTX638548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PELO antibody [HL2345] used in IHC (Paraffin sections) (IHC-P). GTX638548

GTX638548 IHC-P Image

PELO antibody [HL2345] detects PELO protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
PELO stained by PELO antibody [HL2345] (GTX638548) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PELO antibody [HL2345] used in IHC (Paraffin sections) (IHC-P). GTX638548

GTX638548 IHC-P Image

PELO antibody [HL2345] detects PELO protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
PELO stained by PELO antibody [HL2345] (GTX638548) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PELO antibody [HL2345] used in IHC (Paraffin sections) (IHC-P). GTX638548

GTX638548 IHC-P Image

PELO antibody [HL2345] detects PELO protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
PELO stained by PELO antibody [HL2345] (GTX638548) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations