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PELP1 antibody [GT1339]

Recombinant antibody
Anti-PELP1 antibody [GT1339] used in Western Blot (WB). GTX03251
Anti-PELP1 antibody [GT1339] used in Western Blot (WB). GTX03251
Anti-PELP1 antibody [GT1339] used in Western Blot (WB). GTX03251
Anti-PELP1 antibody [GT1339] used in Western Blot (WB). GTX03251

Cat. No. GTX03251

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1339

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all PELP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

120 kDa. ( Note )

Observed MW

160-180 kDa. The observed M.W. is based on the following publication. PMID: 23715282

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PELP1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proline, glutamate and leucine rich protein 1 , MNAR , P160

Cellular Localization

nucleoplasm , Nucleus , Cytoplasm , Nucleolus

Background

This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-PELP1 antibody [GT1339] used in Western Blot (WB). GTX03251

GTX03251 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PELP1 antibody [GT1339] (GTX03251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23715282

Anti-PELP1 antibody [GT1339] used in Western Blot (WB). GTX03251

GTX03251 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PELP1 antibody [GT1339] (GTX03251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23715282

Anti-PELP1 antibody [GT1339] used in Western Blot (WB). GTX03251

GTX03251 WB Image

WB analysis of various samples using GTX03251 PELP1 antibody [GT1339].
Dilution : 1:1000
Loading : 25μg per lane

Anti-PELP1 antibody [GT1339] used in Western Blot (WB). GTX03251

GTX03251 WB Image

WB analysis of rat testis tissue lysate using GTX03251 PELP1 antibody [GT1339].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 439