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PER2 antibody

KO/KD Orthogonal Overexpression
Anti-PER2 antibody used in Western Blot (WB). GTX129688
Anti-PER2 antibody used in Western Blot (WB). GTX129688
Anti-PER2 antibody used in Western Blot (WB). GTX129688
Anti-PER2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129688
Anti-PER2 antibody used in Western Blot (WB). GTX129688
Anti-PER2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129688

Cat. No. GTX129688

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PER2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

137 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PER2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886072

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

period circadian regulator 2 , FASPS , FASPS1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PER2 antibody used in Western Blot (WB). GTX129688

GTX129688 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PER2 antibody used in Western Blot (WB). GTX129688

GTX129688 WB Image

Orthogonal

Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with PER2 antibody (GTX129688) and BMAL1 antibody [N1N3] (GTX105060) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 27834218

Anti-PER2 antibody used in Western Blot (WB). GTX129688

GTX129688 WB Image

Orthogonal

Untreated and treated 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PER2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129688

GTX129688 ICC/IF Image

PER2 antibody detects PER2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PER2 stained by PER2 antibody (GTX129688) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PER2 antibody used in Western Blot (WB). GTX129688

GTX129688 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER2 antibody (GTX129688) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PER2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129688

GTX129688 IHC-P Image

PER2 antibody detects PER2 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: PER2 protein stained by PER2 antibody (GTX129688) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

YY Chiou et al. J Biol Chem 2024; : 105637 Circadian modulation of glucose utilization via CRY1-mediated repression of Pdk1 expression
Application : WB
Ding M et al. Front Physiol 2022; 13 : 1024038 Acute hypoxia induced dysregulation of clock-controlled ovary functions.
Yongkang Zhen et al. Front Immunol 2022; 13 : 848248 Normal Light-Dark and Short-Light Cycles Regulate Intestinal Inflammation, Circulating Short-chain Fatty Acids and Gut Microbiota in Period2 Gene Knockout Mice.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169