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PERK antibody

Anti-PERK antibody used in Western Blot (WB). GTX129275
Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX129275
Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX129275

Cat No. GTX129275

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PERK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 3 , PEK , PERK , WRS

Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PERK antibody used in Western Blot (WB). GTX129275

GTX129275 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PERK antibody (GTX129275) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX129275

GTX129275 IHC-P Image

PERK antibody detects PERK protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PERK stained by PERK antibody (GTX129275) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX129275

GTX129275 IHC-P Image

PERK antibody detects PERK protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PERK stained by PERK antibody (GTX129275) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169