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PERK antibody

Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX66661
Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX66661
Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX66661
Anti-PERK antibody used in Western Blot (WB). GTX66661
Anti-PERK antibody used in Western Blot (WB). GTX66661

Cat. No. GTX66661

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PERK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 56-102 of Human PERK.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 3 , PEK , PERK , WRS

Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX66661

GTX66661 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX66661 PERK antibody.
Left : Primary antibody
Right : Negative control
Dilution : 1:50

Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX66661

GTX66661 IHC-P Image

IHC-P analysis of human esophagus carcinoma tissue using GTX66661 PERK antibody.
Left : Primary antibody
Right : Negative control
Dilution : 1:50

Anti-PERK antibody used in IHC (Paraffin sections) (IHC-P). GTX66661

GTX66661 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX66661 PERK antibody.
Left : Primary antibody
Right : Negative control
Dilution : 1:50

Anti-PERK antibody used in Western Blot (WB). GTX66661

GTX66661 WB Image

WB analysis of various samples using GTX66661 PERK antibody.
Lane1 : HCT116 whole cell lysate(20ug)
Lane2 : MCF-7 whole cell lysate(20ug)
Lane3 : A375 whole cell lysate(20ug)
Lane4 : CT26 whole cell lysate(40ug)
Lane5 : PMVEC whole cell lysate(40ug)
Dilution : 1:1000

Anti-PERK antibody used in Western Blot (WB). GTX66661

GTX66661 WB Image

WB analysis of various samples using GTX66661 PERK antibody.
Lane1 : L02 whole cell lysate(40ug)
Lane2 : A549 whole cell lysate(40ug)
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399