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PERK (phospho Ser555) antibody

Orthogonal
Anti-PERK (phospho Ser555) antibody used in Western Blot (WB). GTX00673
Anti-PERK (phospho Ser555) antibody used in Western Blot (WB). GTX00673
Anti-PERK (phospho Ser555) antibody used in ELISA (ELISA). GTX00673

Cat. No. GTX00673

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($419)
See all PERK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human perk around the phosphorylation site of Ser555.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns

Conjugation

Unconjugated

RRID

AB_2885204

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EIF2AK3 , PEK , PERK , WRS , eukaryotic translation initiation factor 2 alpha kinase 3

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PERK (phospho Ser555) antibody used in Western Blot (WB). GTX00673

GTX00673 WB Image

Orthogonal

WB analysis of HepG2 lysate using GTX00673 PERK (phospho Ser555) antibody preincubated with phospho- and non phospho- peptide.

Anti-PERK (phospho Ser555) antibody used in Western Blot (WB). GTX00673

GTX00673 WB Image

Orthogonal

WB analysis of serum starvation treated EC304 cell lysates using GTX00673 PERK (phospho Ser555) antibody. The lane on the left was treated with blocking peptide.

Anti-PERK (phospho Ser555) antibody used in ELISA (ELISA). GTX00673

GTX00673 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00673 PERK (phospho Ser555) antibody.
P-peptide: phospho-peptide
N-peptide: non-phospho-peptide
Peptides concentration: 1μg/ml

REFERENCE

Application Reference

Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419