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PERK (phospho Ser555) antibody

Anti-PERK (phospho Ser555) antibody used in ELISA (ELISA). GTX00673
Anti-PERK (phospho Ser555) antibody used in Western Blot (WB). GTX00673
Anti-PERK (phospho Ser555) antibody used in Western Blot (WB). GTX00673

Cat No. GTX00673

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human perk around the phosphorylation site of Ser555.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EIF2AK3,PEK,PERK,WRS,eukaryotic translation initiation factor 2 alpha kinase 3

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES
Anti-PERK (phospho Ser555) antibody used in ELISA (ELISA). GTX00673

GTX00673 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00673 PERK (phospho Ser555) antibody.
P-peptide: phospho-peptide
N-peptide: non-phospho-peptide
Peptides concentration: 1μg/ml

Anti-PERK (phospho Ser555) antibody used in Western Blot (WB). GTX00673

GTX00673 WB Image

WB analysis of HepG2 lysate using GTX00673 PERK (phospho Ser555) antibody preincubated with phospho- and non phospho- peptide.

Anti-PERK (phospho Ser555) antibody used in Western Blot (WB). GTX00673

GTX00673 WB Image

WB analysis of serum starvation treated EC304 cell lysates using GTX00673 PERK (phospho Ser555) antibody. The lane on the left was treated with blocking peptide.

REFERENCE
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REVIEW
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Package List Price ($)
$ 319