ICC/IF analysis PFA-fixed A549 cells using GTX04533 PERK (phospho Thr982) antibody.
Permeabilization : 0.1% saponin
Dilution : 1:200

WB analysis of various cell lysates using GTX04533 PERK (phospho Thr982) antibody.
Lane 1 : 293 cells with heat-shock treatment followed by blocking with antigen-specific peptides
Lane 2 : 293 cells with heat-shock treatment
Lane 3 : A549 cells with serum starvation treatment

ICC/IF analysis of PFA-fixed HepG2 cells with 4 μM Forskolin treatment using GTX04533 PERK (phospho Thr982) antibody.
Red : Primary antibody
Green : beta Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

IHC-P analysis of human liver carcinoma tissue using GTX04533 PERK (phospho Thr982) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04533 PERK (phospho Thr982) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/ml