GeneTex

  • Country / Location Selection

United States (US)

PERK (phospho Thr982) antibody

Anti-PERK (phospho Thr982) antibody used in ELISA (ELISA). GTX04533
Anti-PERK (phospho Thr982) antibody used in IHC (Paraffin sections) (IHC-P). GTX04533
Anti-PERK (phospho Thr982) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04533
Anti-PERK (phospho Thr982) antibody used in Western Blot (WB). GTX04533
Anti-PERK (phospho Thr982) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04533

Cat. No. GTX04533

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PERK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-3000
ICC/IF 1:100-500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human EIF2AK3(Accession Q9NZJ5), corresponding to amino acid residues around phosphorylated Thr 982.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 3 , PEK , PERK , WRS

Background

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-PERK (phospho Thr982) antibody used in ELISA (ELISA). GTX04533

GTX04533 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04533 PERK (phospho Thr982) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/ml

Anti-PERK (phospho Thr982) antibody used in IHC (Paraffin sections) (IHC-P). GTX04533

GTX04533 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX04533 PERK (phospho Thr982) antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-PERK (phospho Thr982) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04533

GTX04533 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells with 4 μM Forskolin treatment using GTX04533 PERK (phospho Thr982) antibody.
Red : Primary antibody
Green : beta Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-PERK (phospho Thr982) antibody used in Western Blot (WB). GTX04533

GTX04533 WB Image

WB analysis of various cell lysates using GTX04533 PERK (phospho Thr982) antibody.
Lane 1 : 293 cells with heat-shock treatment followed by blocking with antigen-specific peptides
Lane 2 : 293 cells with heat-shock treatment
Lane 3 : A549 cells with serum starvation treatment

Anti-PERK (phospho Thr982) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04533

GTX04533 ICC/IF Image

ICC/IF analysis PFA-fixed A549 cells using GTX04533 PERK (phospho Thr982) antibody.
Permeabilization : 0.1% saponin
Dilution : 1:200

REFERENCE

There are currently no references for PERK (phospho Thr982) antibody (GTX04533). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PERK (phospho Thr982) antibody (GTX04533). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399