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PEX14 antibody

Anti-PEX14 antibody used in Western Blot (WB). GTX129230
Anti-PEX14 antibody used in Western Blot (WB). GTX129230
Anti-PEX14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129230
Anti-PEX14 antibody used in Western Blot (WB). GTX129230

Cat No. GTX129230

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , HeLa , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEX14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peroxisomal biogenesis factor 14 , NAPP2 , PBD13A , Pex14p , dJ734G22.2

Cellular Localization

Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side

Background

This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PEX14 antibody used in Western Blot (WB). GTX129230

GTX129230 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PEX14 antibody (GTX129230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PEX14 antibody used in Western Blot (WB). GTX129230

GTX129230 WB Image

PEX14 antibody detects PEX14 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
10% SDS-PAGE
PEX14 antibody (GTX129230) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PEX14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129230

GTX129230 ICC/IF Image

PEX14 antibody detects PEX14 protein at peroxisome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PEX14 protein stained by PEX14 antibody (GTX129230) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PEX14 antibody used in Western Blot (WB). GTX129230

GTX129230 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PEX14 antibody (GTX129230) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf