APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
1:50-1:200 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H292 , mouse liver
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PEX19. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888022
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxisomal biogenesis factor 19 , D1S2223E , HK33 , PBD12A , PMP1 , PMPI , PXF , PXMP1
Cellular Localization
Cytoplasm , Peroxisome membrane; Lipid-anchor; Cytoplasmic side
Background
This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX628212 IHC-P Image
PEX19 antibody [GT554] detects PEX19 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. PEX19 antibody [GT554] (GTX628212) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628212 ICC/IF Image
PEX19 antibody [GT554] detects PEX19 protein at cytoplasm by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PEX19 stained by PEX19 antibody [GT554] (GTX628212) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX628212 WB Image
PEX19 antibody [GT554] detects PEX19 protein by Western blot analysis. A. 50 μg mouse liver lysate/extract 10 % SDS-PAGE PEX19 antibody [GT554] (GTX628212) dilution: 1:1000
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GTX628212 FACS Image
PEX19 antibody [GT554] (GTX628212) detects PEX19 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: PEX19 antibody [GT554] (GTX628212) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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GTX628212 WB Image
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 10% SDS PAGE GTX628212 diluted at 1:5000
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REFERENCE
There are currently no references for PEX19 antibody [GT554] (GTX628212). Be the first to share your publications with this product.
REVIEW
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