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PEX19 antibody [GT554]

Anti-PEX19 antibody [GT554] used in IHC (Paraffin sections) (IHC-P). GTX628212
Anti-PEX19 antibody [GT554] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628212
Anti-PEX19 antibody [GT554] used in Western Blot (WB). GTX628212
Anti-PEX19 antibody [GT554] used in Flow cytometry (FACS). GTX628212
Anti-PEX19 antibody [GT554] used in Western Blot (WB). GTX628212

Cat. No. GTX628212

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT554

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PEX19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H292 , mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEX19. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888022

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxisomal biogenesis factor 19 , D1S2223E , HK33 , PBD12A , PMP1 , PMPI , PXF , PXMP1

Cellular Localization

Cytoplasm , Peroxisome membrane; Lipid-anchor; Cytoplasmic side

Background

This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PEX19 antibody [GT554] used in IHC (Paraffin sections) (IHC-P). GTX628212

GTX628212 IHC-P Image

PEX19 antibody [GT554] detects PEX19 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
PEX19 antibody [GT554] (GTX628212) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PEX19 antibody [GT554] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628212

GTX628212 ICC/IF Image

PEX19 antibody [GT554] detects PEX19 protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PEX19 stained by PEX19 antibody [GT554] (GTX628212) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PEX19 antibody [GT554] used in Western Blot (WB). GTX628212

GTX628212 WB Image

PEX19 antibody [GT554] detects PEX19 protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
10 % SDS-PAGE
PEX19 antibody [GT554] (GTX628212) dilution: 1:1000

Anti-PEX19 antibody [GT554] used in Flow cytometry (FACS). GTX628212

GTX628212 FACS Image

PEX19 antibody [GT554] (GTX628212) detects PEX19 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: PEX19 antibody [GT554] (GTX628212) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-PEX19 antibody [GT554] used in Western Blot (WB). GTX628212

GTX628212 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
E: HL-60
F: NCI-H929
10% SDS PAGE
GTX628212 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations