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PEX19 antibody

Orthogonal
Anti-PEX19 antibody used in IHC (Paraffin sections) (IHC-P). GTX110721
Anti-PEX19 antibody used in Western Blot (WB). GTX110721
Anti-PEX19 antibody used in Western Blot (WB). GTX110721
Anti-PEX19 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110721
Anti-PEX19 antibody used in Western Blot (WB). GTX110721
Anti-PEX19 antibody used in Western Blot (WB). GTX110721
Anti-PEX19 antibody used in IHC (Paraffin sections) (IHC-P). GTX110721

Cat. No. GTX110721

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PEX19 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

mouse liver , PC-12 , Rat-2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEX19. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951172

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxisomal biogenesis factor 19 , D1S2223E , HK33 , PBD12A , PMP1 , PMPI , PXF , PXMP1

Cellular Localization

Cytoplasm , Peroxisome membrane; Lipid-anchor; Cytoplasmic side

Background

This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PEX19 antibody used in IHC (Paraffin sections) (IHC-P). GTX110721

GTX110721 IHC-P Image

PEX19 antibody detects PEX19 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
PEX19 stained by PEX19 antibody (GTX110721) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PEX19 antibody used in Western Blot (WB). GTX110721

GTX110721 WB Image

PEX19 antibody detects PEX19 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10% SDS-PAGE
PEX19 antibody (GTX110721) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PEX19 antibody used in Western Blot (WB). GTX110721

GTX110721 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX110721 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PEX19 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110721

GTX110721 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PEX19(GTX110721) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-PEX19 antibody used in Western Blot (WB). GTX110721

GTX110721 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PEX19 antibody (GTX110721) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PEX19 antibody used in Western Blot (WB). GTX110721

GTX110721 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PEX19 antibody (GTX110721) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PEX19 antibody used in IHC (Paraffin sections) (IHC-P). GTX110721

GTX110721 IHC-P Image

PEX19 antibody detects PEX19 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PEX19 stained by PEX19 antibody (GTX110721) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wilhelm LP et al. EMBO J 2022; 41 (24) : e111115 BNIP3L/NIX regulates both mitophagy and pexophagy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169