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PEX5 antibody

Anti-PEX5 antibody used in Western Blot (WB). GTX109798
Anti-PEX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109798
Anti-PEX5 antibody used in Western Blot (WB). GTX109798

Cat. No. GTX109798

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PEX5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PEX5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951175

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxisomal biogenesis factor 5 , PBD2A , PBD2B , PTS1-BP , PTS1R , PXR1 , RCDP5

Cellular Localization

Cytoplasm , Peroxisome membrane; Peripheral membrane protein

Background

The product of this gene binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import. Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of neonatal adrenoleukodystrophy (NALD), a cause of Zellweger syndrome (ZWS) as well as may be a cause of infantile Refsum disease (IRD). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PEX5 antibody used in Western Blot (WB). GTX109798

GTX109798 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX109798 diluted at 1:5000

Anti-PEX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109798

GTX109798 IHC-P Image

PEX5 antibody detects PEX5 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
PEX5 antibody (GTX109798) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PEX5 antibody used in Western Blot (WB). GTX109798

GTX109798 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PEX5 antibody (GTX109798) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sang-Ah Lee et al. Mol Cells 2021; 44 (4) : 214-222 The Peroxisomal Localization of Hsd17b4 Is Regulated by Its Interaction with Phosphatidylserine.
Application : WB
Reactivity : Mouse
Mu Y et al. PLoS One 2021; 16 (2) : e0245799 Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell.
Application : WB
Reactivity : Mouse
Eun SY et al. Exp Mol Med 2018; 50 (4) : 4 PEX5 regulates autophagy via the mTORC1-TFEB axis during starvation.
Application : WB
Reactivity : Human
Mizuno Y et al. PLoS Genet 2013; 9 (2) : e1003286 Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.
Application : WB
Reactivity : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169