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PFDN3 antibody

Anti-PFDN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114875
Anti-PFDN3 antibody used in Western Blot (WB). GTX114875
Anti-PFDN3 antibody used in Western Blot (WB). GTX114875

Cat. No. GTX114875

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PFDN3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Mouse brain , A549

Predict Reactivity

Rabbit, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFDN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619124

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VHL binding protein 1 , HIBBJ46 , PFD3 , PFDN3 , VBP-1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PFDN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114875

GTX114875 ICC/IF Image

VBP1 antibody detects VBP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VBP1 protein stained by VBP1 antibody (GTX114875) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-PFDN3 antibody used in Western Blot (WB). GTX114875

GTX114875 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114875 diluted at 1:1000

Anti-PFDN3 antibody used in Western Blot (WB). GTX114875

GTX114875 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX114875 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169