GeneTex
United States (US)

PFDN3 antibody

GTX114875_WB_18121410_741.jpg
GTX114875_40240_WB_M_brain_18121410_525.jpg
GTX114875_40240_20150410_IFA_18121410_127.jpg

Cat No. GTX114875

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

A549 , mouse brain

Predict Reactivity

Rabbit, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFDN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PFD3 antibody, PFDN3 antibody, VBP-1 antibody, VBP1 antibody, VHL binding protein-1 antibody, von Hippel-Lindau-binding protein 1 antibody, prefoldin 3 antibody, HIBBJ46 antibody, prefoldin subunit 3 antibody, VHL-binding protein 1 antibody, von Hippel-Lindau binding protein 1 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. [provided by RefSeq]
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Database

DATA IMAGES
GTX114875_WB_18121410_741.jpg

GTX114875 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX114875 diluted at 1:1000

GTX114875_40240_WB_M_brain_18121410_525.jpg

GTX114875 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114875 diluted at 1:1000

GTX114875_40240_20150410_IFA_18121410_127.jpg

GTX114875 ICC/IF Image

VBP1 antibody detects VBP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VBP1 protein stained by VBP1 antibody (GTX114875) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169