*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , H1299 , HeLa , Molt-4 , Raji
Mouse, Rat, Bovine, Pig(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.67 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human PFKFB1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 , F6PK , HL2K , PFRX
This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq]
GTX111733 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using PFKFB1(GTX111733) antibody at 1:200 dilution.
GTX111733 IHC-P Image
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using PFKFB1(GTX111733) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX111733 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB1 antibody [N1N3] (GTX111733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.