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PFKFB1 antibody [N1N3]

Anti-PFKFB1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111733
Anti-PFKFB1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111733
Anti-PFKFB1 antibody [N1N3] used in Western Blot (WB). GTX111733

Cat No. GTX111733

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all PFKFB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

293T , H1299 , HeLa , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKFB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951177

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 , F6PK , HL2K , PFRX

Background

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PFKFB1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111733

GTX111733 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PFKFB1(GTX111733) antibody at 1:200 dilution.

Anti-PFKFB1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111733

GTX111733 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using PFKFB1(GTX111733) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKFB1 antibody [N1N3] used in Western Blot (WB). GTX111733

GTX111733 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB1 antibody [N1N3] (GTX111733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169