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PFKFB2 antibody

Anti-PFKFB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55273
Anti-PFKFB2 antibody used in Western Blot (WB). GTX55273
Anti-PFKFB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX55273

Cat No. GTX55273

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

58 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of PFKFB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 2,Pfk-2/Fbpase-2,Pfkfb2

Background

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-PFKFB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55273

GTX55273 ICC/IF Image

ICC/IF analysis of formalin-fixed HepG2 cells using GTX55273 PFKFB2 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-PFKFB2 antibody used in Western Blot (WB). GTX55273

GTX55273 WB Image

WB analysis of HCT116 (A), NIH3T3 (B) whole cell lysates using GTX55273 PFKFB2 antibody.

Anti-PFKFB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX55273

GTX55273 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX55273 PFKFB2 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE
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Package List Price ($)
$ 319