ICC/IF analysis of formalin-fixed HepG2 cells using GTX55273 PFKFB2 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

WB analysis of HCT116 (A), NIH3T3 (B) whole cell lysates using GTX55273 PFKFB2 antibody.

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX55273 PFKFB2 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)