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PFKFB4 antibody [HL2204]

Recombinant antibody Orthogonal Overexpression
Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208
Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208
Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208
Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208
Anti-PFKFB4 antibody [HL2204] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638208
Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208
Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208
Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208

Cat. No. GTX638208

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2204

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PFKFB4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This antibody is specific for human PFKFB4 protein, and it does not cross react with human PFKFB1, PFKFB2, and PFKFB3 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human PFKFB4.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4

Background

The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208

GTX638208 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208

GTX638208 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208

GTX638208 IHC-P Image

PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKFB4 antibody [HL2204] used in IHC (Paraffin sections) (IHC-P). GTX638208

GTX638208 IHC-P Image

PFKFB4 antibody [HL2204] detects PFKFB4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKFB4 antibody [HL2204] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638208

GTX638208 ICC/IF Image

PFKFB4 antibody [HL2204] detects PFKFB4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.
Green: PFKFB4 stained by PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208

GTX638208 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208

GTX638208 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKFB4 antibody [HL2204] used in Western Blot (WB). GTX638208

GTX638208 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody [HL2204] (GTX638208) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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Package List Price ($)
$ 399
$ 169
Custom Conjugations