GeneTex
United States (US)

PFKFB4 antibody [N3C3]

GTX106237_40002_IHC_M_19011506_956.jpg
GTX106237_WB_19011506_959.jpg

Cat No. GTX106237

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKFB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 4,Pfkfb4

Background

Synthesis and degradation of fructose 2,6-bisphosphate.

Database

Research Area

DATA IMAGES
GTX106237_40002_IHC_M_19011506_956.jpg

GTX106237 IHC-P Image

PFKFB4 antibody [N3C3] detects PFKFB4 protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PFKFB4 antibody [N3C3] (GTX106237) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106237_WB_19011506_959.jpg

GTX106237 WB Image

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
10% SDS PAGE
GTX106237 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf