GeneTex
United States (US)

PFKFB4 antibody [N3C3]

GTX106237_WB_19011506_959.jpg
GTX106237_40002_IHC_M_19011506_956.jpg

Cat No. GTX106237

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKFB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PFKFB4 antibody, "6PF-2-K/Fru-2,6-P2ase testis-type isozyme antibody", PFK/FBPase 4 antibody, "6PF-2-K/Fru-2,6-P2ase 4 antibody", "6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 antibody", "6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4 antibody", bifunctional enzyme with kinase and biphosphatase activities antibody

Background

Synthesis and degradation of fructose 2,6-bisphosphate.

Database

Research Area

DATA IMAGES
GTX106237_WB_19011506_959.jpg

GTX106237 WB Image

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
B:Raji (GTX27908)
10% SDS PAGE
GTX106237 diluted at 1:500

GTX106237_40002_IHC_M_19011506_956.jpg

GTX106237 IHC-P Image

PFKFB4 antibody [N3C3] detects PFKFB4 protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PFKFB4 antibody [N3C3] (GTX106237) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf