GeneTex
United States (US)

PFKFB4 antibody

GTX107755_WB_19011507_173.jpg
GTX107755_39981_20150206_IHC_M_2_19011507_292.jpg
GTX107755_39981_IHC_19011507_504.jpg
GTX107755_42172_20160105_IHC-P_M_19011507_932.jpg
GTX107755_41066_IFA_19011507_309.jpg

Cat No. GTX107755

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Raji , mouse liver , rat liver

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKFB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 4,Pfkfb4

Background

Synthesis and degradation of fructose 2,6-bisphosphate.

Database

Research Area

DATA IMAGES
GTX107755_WB_19011507_173.jpg

GTX107755 WB Image

Sample(30 µg whole cell lysate)
A:Raji (GTX27908)
10% SDS PAGE
GTX107755 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107755_39981_20150206_IHC_M_2_19011507_292.jpg

GTX107755 IHC-P Image

PFKFB4 antibody detects PFKFB4 protein at cytoplasm on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PFKFB4 antibody (GTX107755) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107755_39981_IHC_19011507_504.jpg

GTX107755 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PFKFB4(GTX107755) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107755_42172_20160105_IHC-P_M_19011507_932.jpg

GTX107755 IHC-P Image

PFKFB4 antibody detects PFKFB4 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PFKFB4 antibody (GTX107755) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107755_41066_IFA_19011507_309.jpg

GTX107755 ICC/IF Image

PFKFB4 antibody detects PFKFB4 protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: PFKFB4 protein stained by PFKFB4 antibody (GTX107755) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf