GeneTex

  • Country / Location Selection

United States (US)

PFKFB4 antibody

Anti-PFKFB4 antibody used in Western Blot (WB). GTX107755
Anti-PFKFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX107755
Anti-PFKFB4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107755
Anti-PFKFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX107755
Anti-PFKFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX107755
Anti-PFKFB4 antibody used in Western Blot (WB). GTX107755

Cat. No. GTX107755

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PFKFB4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKFB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951183

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4

Background

Synthesis and degradation of fructose 2,6-bisphosphate.

Database

Research Area

DATA IMAGES

Anti-PFKFB4 antibody used in Western Blot (WB). GTX107755

GTX107755 WB Image

PFKFB4 antibody detects PFKFB4 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PFKFB4 antibody (GTX107755) diluted at 1:2000.

Anti-PFKFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX107755

GTX107755 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PFKFB4(GTX107755) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PFKFB4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107755

GTX107755 ICC/IF Image

PFKFB4 antibody detects PFKFB4 protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: PFKFB4 protein stained by PFKFB4 antibody (GTX107755) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PFKFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX107755

GTX107755 IHC-P Image

PFKFB4 antibody detects PFKFB4 protein at cytoplasm on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
PFKFB4 antibody (GTX107755) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PFKFB4 antibody used in IHC (Paraffin sections) (IHC-P). GTX107755

GTX107755 IHC-P Image

PFKFB4 antibody detects PFKFB4 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PFKFB4 antibody (GTX107755) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKFB4 antibody used in Western Blot (WB). GTX107755

GTX107755 WB Image

Sample(30 μg whole cell lysate)
A:Raji (GTX27908)
10% SDS PAGE
GTX107755 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Carla Proserpio et al. Cancers (Basel) 2022; 14 (9) MEOX2 Regulates the Growth and Survival of Glioblastoma Stem Cells by Modulating Genes of the Glycolytic Pathway and Response to Hypoxia.
Application : WB
Reactivity : Human
Hsin MC et al. Cancers (Basel) 2021; 13 (5) Carbonic Anhydrase IX Promotes Human Cervical Cancer Cell Motility by Regulating PFKFB4 Expression.
Application : WB
Reactivity : Human
Hsin M.C. et al. Cancers 2021; 13 (5) : 1174 Carbonic Anhydrase IX Promotes Human Cervical Cancer Cell Motility by Regulating PFKFB4 Expression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PFKFB4 antibody (GTX107755). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169